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lambda isolated human myeloma plasma (Millipore)

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Millipore igg1/kappa or 156 igg1/lambda isolated human myeloma plasma
Igg1/Kappa Or 156 Igg1/Lambda Isolated Human Myeloma Plasma, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+igg1+lambda/anti+map2/ppr0425917-64-2-13
Average 90 stars, based on 1 article reviews

igg1/kappa or 156 igg1/lambda isolated human myeloma plasma - by Bioz Stars, 2026-10

90/100 stars

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Binding Assay:

Article Title: Pemphigus Vulgaris and Foliaceus IgG Autoantibodies Directly Block Heterophilic Transinteraction between Desmoglein and Desmocollin.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

other:

Article Title: Aggregation risk prediction for antibodies and its application to biotherapeutic development
Article Snippet: 100 μl of standard or sample was loaded per well and incubated at room temperature with shaking at 300 rpm for 1 h. Plates were washed as described previously and 100 μl of suitably diluted Anti-human IgG kappa HRP (Caltag) or Anti-human IgG Lambda HRP (Sigma-Aldrich) added and incubated at room temperature with shaking at 300 rpm for 1 h. The plate was washed again (3 × 250 μl per well) and 100 μl of 3,3,5,5‘-tetramethylbenzidine (TMB) substrate (Sigma-Aldrich) was added and incubated at room temperature for 15 min (Lambda) or 10 min (Kappa).

Article Title: Enrichment of Antigen-Specific Class-Switched B Cells from Individuals Naturally Immunized by Infection with Group A Streptococcus
Article Snippet: Human IgG1-lambda (Sigma-Aldrich, St. Louis, MO) was used as a standard.

Activation Assay:

Article Title: Soluble hybrid Fcγ receptors and related methods
Article Snippet: Measurements were performed using a Biacore 3000 instrument (Piscataway, N.J.). .. Activation of the sensor chip surface and covalent immobilization of the IgG1 antibody (Lambda from human myeloma plasma, Sigma-Aldrich, St Louis, Mo.) was performed using 0.2 M N-ethyl-N′-(3-diethylamino-propyl) carbodiimide and 0.05 M N-hydroxysuccinamide and the Biacore Control Software. ..

Clinical Proteomics:

Article Title: Recombinant neutralizing secretory IgA antibodies for preventing mucosal acquisition and transmission of SARS-CoV-2.
Article Snippet: .. As standards, IgG1/kappa or 493 IgG1/lambda isolated from human myeloma plasma (15154, I5029, Sigma, US), purified 494 human IgA (P80-102, Bethyl, US) and IgA from human colostrum (I2363, Sigma, US) were 495 used. .. Detection of secretory IgA variants was carried out with mouse anti-secretory component 496 antibody (SAB4200787, Sigma, US), followed by HRP-labeled anti-mouse antibody 497 (SAB5300168, Sigma, US).

Control:

Software:

Recombinant:

Article Title: Immuno-Northern Blotting: Detection of RNA Modifications by Using Antibodies against Modified Nucleosides
Article Snippet: .. Other antibodies and materials were purchased as follows: rabbit polyclonal anti- N 6-methyladenosine antibody (Synaptic Systems), horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Thermo), HRP-conjugated goat anti-rabbit IgG (Thermo), mouse IgG2a lambda (Sigma-Aldrich), DNase I (RNase-free, Qiagen), RNase inhibitor (Roche), sodium meta-arsenite (Wako), recombinant human FTO protein (Abcam), and recombinant human ALKBH5 protein (Abnova). ..

Isolation:

Purification:

Image Search Results

(A) Flow cytometry gating strategies to identify B-cell populations in LN cells derived from SHIV-infected RMs. (B) Alluvial plots showing percentages of IgG + GC, IgG + Bmem and IgM + Bmem populations among CD20 + LN cells during the infection time course in the three RMs in each infection group. (C-E) Alluvial plots showing percentages of SOSIP-binding (C), gp140-binding (D), and autologous neutralizing (E) IgG + single B-cell cultures from different B-cell populations over the infection course. See also Figures S1 and S2, and Tables S1 and S2.

Journal: bioRxiv

Article Title: Functional Convergence of Genetically Diverse B-Cell Receptors in Simian-HIV Infected Rhesus Macaques

doi: 10.64898/2026.01.09.698730

Figure Lengend Snippet: (A) Flow cytometry gating strategies to identify B-cell populations in LN cells derived from SHIV-infected RMs. (B) Alluvial plots showing percentages of IgG + GC, IgG + Bmem and IgM + Bmem populations among CD20 + LN cells during the infection time course in the three RMs in each infection group. (C-E) Alluvial plots showing percentages of SOSIP-binding (C), gp140-binding (D), and autologous neutralizing (E) IgG + single B-cell cultures from different B-cell populations over the infection course. See also Figures S1 and S2, and Tables S1 and S2.

Article Snippet: For human IgG ELISA assays, the coating antibodies used were goat anti-human Igκ (2 μg/ml, polyclonal, Southern Biotech, Cat# 2061-01) and goat anti-human Igλ (2 μg/ml, polyclonal, Southern Biotech, Cat# 2071-01), the standard antibodies used were human IgG1 Kappa (polyclonal, Southern Biotech, Cat# 0151K-01) and human IgG1 Lambda (polyclonal, Southern Biotech, Cat# 0151L-01), and the detection antibody used was goat anti-human IgG-HRP (1/5000 dilution, polyclonal, Southern Biotech, Cat# 2040-05).

Techniques: Flow Cytometry, Derivative Assay, Infection, Binding Assay

(A) Kinetics of IgG production in culture supernatants from RM IgM + and IgG + single B cells cultured with the MS40L feeder cell line. Each dot represents an individual IgG + culture well. Dashed lines indicate detection limits, and error bars (in red) represent the median values. (B-C) Comparison of the original MS40L and engineered MEC-147 feeder cell lines in supporting single B-cell cultures from different B-cell populations. Panel (B) shows the culture of B cells isolated from the lymph nodes of an RM after DTaP immunization, while panel (C) shows the culture of B cells isolated from PBMCs of a healthy human donor. The top panels display the percentage of IgG + culture wells among all single-cell sorted wells. Error bars indicate the mean + SD across three 96-well culture plates. The bottom panels show OD450 values from IgG ELISA assays, with each dot representing an individual IgG + culture well. Dashed lines indicate detection limits, and error bars (in red) represent the median values. (D) Single nucleated cells were isolated from LN biopsies of infected RMs at various time points following SHIV infection. B cells of different phenotypes, including mature follicular (MF), IgM + Bmem, IgG + GC, and IgG + Bmem cells, were identified from isolated LN cells by flow cytometry and sorted into single B-cell cultures with MEC-147 feeder cells. Supernatants were harvested after 18 days of culture, and IgG-producing cultures were identified using standard ELISA assays. IgG-containing culture supernatants were tested for neutralization activity against autologous SHIV pseudo-viruses using standard TZM-bl assays. Each dot represents an individual single B-cell culture supernatant containing clonal IgG. Error bars in red indicate median values, while blue dashed lines indicate cutoff thresholds for defining neutralizing versus non-neutralizing cultures. Inserted red numbers represent the percentage of neutralizing cultures among IgG + cultures for the corresponding B-cell populations (see Table S2 for details).

Journal: bioRxiv

Article Title: Functional Convergence of Genetically Diverse B-Cell Receptors in Simian-HIV Infected Rhesus Macaques

doi: 10.64898/2026.01.09.698730

Figure Lengend Snippet: (A) Kinetics of IgG production in culture supernatants from RM IgM + and IgG + single B cells cultured with the MS40L feeder cell line. Each dot represents an individual IgG + culture well. Dashed lines indicate detection limits, and error bars (in red) represent the median values. (B-C) Comparison of the original MS40L and engineered MEC-147 feeder cell lines in supporting single B-cell cultures from different B-cell populations. Panel (B) shows the culture of B cells isolated from the lymph nodes of an RM after DTaP immunization, while panel (C) shows the culture of B cells isolated from PBMCs of a healthy human donor. The top panels display the percentage of IgG + culture wells among all single-cell sorted wells. Error bars indicate the mean + SD across three 96-well culture plates. The bottom panels show OD450 values from IgG ELISA assays, with each dot representing an individual IgG + culture well. Dashed lines indicate detection limits, and error bars (in red) represent the median values. (D) Single nucleated cells were isolated from LN biopsies of infected RMs at various time points following SHIV infection. B cells of different phenotypes, including mature follicular (MF), IgM + Bmem, IgG + GC, and IgG + Bmem cells, were identified from isolated LN cells by flow cytometry and sorted into single B-cell cultures with MEC-147 feeder cells. Supernatants were harvested after 18 days of culture, and IgG-producing cultures were identified using standard ELISA assays. IgG-containing culture supernatants were tested for neutralization activity against autologous SHIV pseudo-viruses using standard TZM-bl assays. Each dot represents an individual single B-cell culture supernatant containing clonal IgG. Error bars in red indicate median values, while blue dashed lines indicate cutoff thresholds for defining neutralizing versus non-neutralizing cultures. Inserted red numbers represent the percentage of neutralizing cultures among IgG + cultures for the corresponding B-cell populations (see Table S2 for details).

Techniques: Cell Culture, Comparison, Isolation, Enzyme-linked Immunosorbent Assay, Infection, Flow Cytometry, Neutralization, Activity Assay

The same IgG-containing single B-cell culture supernatants prepared as described in were tested for binding activity against autologous SOSIP and gp140 antigens using standard Luminex assays, conducted in parallel and in a blinded manner to the autologous neutralization assays shown in . RMs were infected with either the BG505 (A) or CH505 (B) SHIV strains. B-cell populations are color-coded as indicated in the keys. Dashed lines indicate cutoff thresholds for defining antigen-binding versus non-binding cultures. Inserted numbers represent the percentage of antigen-binding cultures among IgG + cultures for the corresponding B-cell populations. Additional antigens or capture antibodies, including CH505.gp120, MN.gp41, anti-RhIgG, and non-relevant controls (streptavidin, BSA, and ovalbumin), were included in the Luminex bead panel (see Table S2 for details).

Journal: bioRxiv

Article Title: Functional Convergence of Genetically Diverse B-Cell Receptors in Simian-HIV Infected Rhesus Macaques

doi: 10.64898/2026.01.09.698730

Figure Lengend Snippet: The same IgG-containing single B-cell culture supernatants prepared as described in were tested for binding activity against autologous SOSIP and gp140 antigens using standard Luminex assays, conducted in parallel and in a blinded manner to the autologous neutralization assays shown in . RMs were infected with either the BG505 (A) or CH505 (B) SHIV strains. B-cell populations are color-coded as indicated in the keys. Dashed lines indicate cutoff thresholds for defining antigen-binding versus non-binding cultures. Inserted numbers represent the percentage of antigen-binding cultures among IgG + cultures for the corresponding B-cell populations. Additional antigens or capture antibodies, including CH505.gp120, MN.gp41, anti-RhIgG, and non-relevant controls (streptavidin, BSA, and ovalbumin), were included in the Luminex bead panel (see Table S2 for details).

Techniques: Cell Culture, Binding Assay, Activity Assay, Luminex, Neutralization, Infection

(A) Related to Table S3. Proportions of identified autologous SOSIP- or gp140-binding B cells for which BCR sequences were determined. The blue horizontal line separates two categories: cultures with binding MFI > 300 (upper) and MFI ≤ 300 (lower). Stacked bars represent proportions normalized to the total counts of Env-binding B cells with MFI > 300, with pink indicating sequenced and grey indicating not sequenced. Numbers within bars denote the counts of Env-binding B cells in each category. (B) Related to . VH mutation frequency and neutralization differences between Bmem and IgG + GC B cells for lineages containing both IgG + GC and Bmem members at the same timepoints in individual RMs. Each dot represents a Bmem–IgG + GC B cell pair. Only RM–Bmem population groups with more than two data points are shown. (C) Related to . Relationship between rAb binding MFI values to heterologous gp140 protein and the binding MFI values of corresponding single B-cell culture supernatants to CH505.gp120 and MN.gp41 proteins in the initial screening. Functional types of source B-cell clones are color-coded, and dashed lines indicate binding cutoffs. (D) Related to Methods. UMAP plots showing the same coordinates and clusters as in , with colors indicating individual assay batches. (E-F) Related to . Normalized density plots display the distribution of distances calculated from the pairwise IGV gene distance matrix at two categorical levels: IGV gene (E) and family (F). Distances within the same category (“Within”) are shown in black, while distances between different categories (“Between”) are shown in grey. Dashed vertical lines in blue represent cutoff values, determined either at the intersection of within and between distance distributions or at the point of minimum difference when no intersection is observed. (G) Related to . Distribution of MADM of IGV allele usage frequencies across RMs, calculated from percentages smoothed within IGV families. The distribution for uCDR3 represents the combined MADM values from 1000 multinomial down-sampled simulations (see Methods). Empirical p values were calculated between the median MADM of xBCR and 1000 median values of randomly-sampled and size-matched uCDR3 subsets (see Methods). ***, empirical p < 0.001. Alleles with zero frequencies across all three RMs were excluded to prevent distortion by an excess of MADM = 0 values. (H) Related to . Cosine similarity scores of IGV allele usage between RMs. For the uCDR3 source, dots indicate medians from 1000 multinomial down-sampling simulations (see Methods), with error bars showing 25th – 75th percentiles. Lines connect the same RM pair comparisons from uCDR3 or xBCR sources, with solid lines for original percentages and dashed lines for percentages smoothed within IGV families. Line colors indicate the significance of empirical p values: grey (***) for p < 0.001, blue (**) for p < 0.01, green (*) for p < 0.05, and orange (ns) for not significant.

Journal: bioRxiv

Article Title: Functional Convergence of Genetically Diverse B-Cell Receptors in Simian-HIV Infected Rhesus Macaques

doi: 10.64898/2026.01.09.698730

Figure Lengend Snippet: (A) Related to Table S3. Proportions of identified autologous SOSIP- or gp140-binding B cells for which BCR sequences were determined. The blue horizontal line separates two categories: cultures with binding MFI > 300 (upper) and MFI ≤ 300 (lower). Stacked bars represent proportions normalized to the total counts of Env-binding B cells with MFI > 300, with pink indicating sequenced and grey indicating not sequenced. Numbers within bars denote the counts of Env-binding B cells in each category. (B) Related to . VH mutation frequency and neutralization differences between Bmem and IgG + GC B cells for lineages containing both IgG + GC and Bmem members at the same timepoints in individual RMs. Each dot represents a Bmem–IgG + GC B cell pair. Only RM–Bmem population groups with more than two data points are shown. (C) Related to . Relationship between rAb binding MFI values to heterologous gp140 protein and the binding MFI values of corresponding single B-cell culture supernatants to CH505.gp120 and MN.gp41 proteins in the initial screening. Functional types of source B-cell clones are color-coded, and dashed lines indicate binding cutoffs. (D) Related to Methods. UMAP plots showing the same coordinates and clusters as in , with colors indicating individual assay batches. (E-F) Related to . Normalized density plots display the distribution of distances calculated from the pairwise IGV gene distance matrix at two categorical levels: IGV gene (E) and family (F). Distances within the same category (“Within”) are shown in black, while distances between different categories (“Between”) are shown in grey. Dashed vertical lines in blue represent cutoff values, determined either at the intersection of within and between distance distributions or at the point of minimum difference when no intersection is observed. (G) Related to . Distribution of MADM of IGV allele usage frequencies across RMs, calculated from percentages smoothed within IGV families. The distribution for uCDR3 represents the combined MADM values from 1000 multinomial down-sampled simulations (see Methods). Empirical p values were calculated between the median MADM of xBCR and 1000 median values of randomly-sampled and size-matched uCDR3 subsets (see Methods). ***, empirical p < 0.001. Alleles with zero frequencies across all three RMs were excluded to prevent distortion by an excess of MADM = 0 values. (H) Related to . Cosine similarity scores of IGV allele usage between RMs. For the uCDR3 source, dots indicate medians from 1000 multinomial down-sampling simulations (see Methods), with error bars showing 25th – 75th percentiles. Lines connect the same RM pair comparisons from uCDR3 or xBCR sources, with solid lines for original percentages and dashed lines for percentages smoothed within IGV families. Line colors indicate the significance of empirical p values: grey (***) for p < 0.001, blue (**) for p < 0.01, green (*) for p < 0.05, and orange (ns) for not significant.

Techniques: Binding Assay, Mutagenesis, Neutralization, Cell Culture, Functional Assay, Clone Assay, Sampling

(A) Expansion/contraction of Env-reactive neutralizing and non-neutralizing IgG + GC B-cell clones in each infection group over time. ND, not detected. (B-C) Distributions of timepoint occurrence (B) and clone size (C) were compared between neutralizing (Neut) and non-neutralizing (NonN) clones. Two-sided Wilcoxon rank-sum tests at the clone level (pooled across RMs within each SHIV group) were used. (D) Clone counts of neutralizing and non-neutralizing IgG + GC B-cell clones in individual RMs. (E) IGHV mutation frequency changes in Env-reactive clones spanning multiple timepoints. All nine clones (named “RM_Clone-ID”) that persisted across at least two timepoints and contained more than two clonal members at each timepoint are shown. Adjacent timepoints were compared using two-sided Wilcoxon rank-sum tests. Statistical annotations: *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, not significant. See also Table S3 and Document S1.

Journal: bioRxiv

Article Title: Functional Convergence of Genetically Diverse B-Cell Receptors in Simian-HIV Infected Rhesus Macaques

doi: 10.64898/2026.01.09.698730

Figure Lengend Snippet: (A) Expansion/contraction of Env-reactive neutralizing and non-neutralizing IgG + GC B-cell clones in each infection group over time. ND, not detected. (B-C) Distributions of timepoint occurrence (B) and clone size (C) were compared between neutralizing (Neut) and non-neutralizing (NonN) clones. Two-sided Wilcoxon rank-sum tests at the clone level (pooled across RMs within each SHIV group) were used. (D) Clone counts of neutralizing and non-neutralizing IgG + GC B-cell clones in individual RMs. (E) IGHV mutation frequency changes in Env-reactive clones spanning multiple timepoints. All nine clones (named “RM_Clone-ID”) that persisted across at least two timepoints and contained more than two clonal members at each timepoint are shown. Adjacent timepoints were compared using two-sided Wilcoxon rank-sum tests. Statistical annotations: *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, not significant. See also Table S3 and Document S1.

Techniques: Clone Assay, Infection, Mutagenesis

(A) Proportions of neutralizing and non-neutralizing Env-reactive B cells from different populations. Inserted numbers indicate cell counts. ND, not detected. (B) Clonal relationship between Env-reactive IgG + GC and Bmem cells. Top sectors show B-cell populations (color-coded); lower sectors represent individual neutralizing or non-neutralizing clones, including lineages and singletons. Color gradients (red for neutralizing and blue for non-neutralizing) were used solely to visually distinguish adjacent individual clones. (C) Venn diagrams showing the distribution of B-cell clones across single or multiple populations. Blank regions indicate zero counts. (D-E) For lineages containing both IgG + GC and Bmem members at the same timepoints: (D) VH mutation frequencies and neutralization activities for all seven RM_Clone-ID_Timepoint groups identified with >3 clonal members; (E) VH mutation frequency and neutralization differences between Bmem and IgG + GC B cells for all 19 RM_Clone-ID_Timepoint groups identified. Each dot represents a Bmem–IgG+ GC B cell pair. See also Table S3.

Journal: bioRxiv

Article Title: Functional Convergence of Genetically Diverse B-Cell Receptors in Simian-HIV Infected Rhesus Macaques

doi: 10.64898/2026.01.09.698730

Figure Lengend Snippet: (A) Proportions of neutralizing and non-neutralizing Env-reactive B cells from different populations. Inserted numbers indicate cell counts. ND, not detected. (B) Clonal relationship between Env-reactive IgG + GC and Bmem cells. Top sectors show B-cell populations (color-coded); lower sectors represent individual neutralizing or non-neutralizing clones, including lineages and singletons. Color gradients (red for neutralizing and blue for non-neutralizing) were used solely to visually distinguish adjacent individual clones. (C) Venn diagrams showing the distribution of B-cell clones across single or multiple populations. Blank regions indicate zero counts. (D-E) For lineages containing both IgG + GC and Bmem members at the same timepoints: (D) VH mutation frequencies and neutralization activities for all seven RM_Clone-ID_Timepoint groups identified with >3 clonal members; (E) VH mutation frequency and neutralization differences between Bmem and IgG + GC B cells for all 19 RM_Clone-ID_Timepoint groups identified. Each dot represents a Bmem–IgG+ GC B cell pair. See also Table S3.

Techniques: Clone Assay, Mutagenesis, Neutralization

(A) Binding activities of reference bnAbs and nnAbs (human IgG1 format) for BG505 and CH505 SOSIP and gp140 antigens were tested in standard Luminex assays using serial dilutions. Vertical dashed lines indicate the concentration point (206 ng/ml) used for calculating MFI ratios in . (B) Binding activities of rAbs (rhesus IgG1 format) derived from representative B-cell clones of different functional types were similarly tested. Results for selected rAbs from BG505-infected (top row) and CH505-infected RMs (bottom row) are shown (named as “RM_Clone-ID (Type)”). Full data for all 146 rAbs are provided in Document S3. Antigens are color-coded as in panel (A). Dashed grey curves represent MFI values detected with Luminex beads conjugated to an anti-RhIgG capture antibody. Vertical dashed lines indicate the concentration points where anti-RhIgG MFI values align closely with those detected using culture supernatants during initial screening. (C) Comparison of MFI values for autologous SOSIP and gp140 antigens detected using culture supernatants (MFI (sup)) during initial screening and rAbs (MFI (rAb)) at selected concentration points (indicated by vertical dashed lines in panel (B) and Document S3). Linear regression models were fitted, and R 2 values indicating the proportion of variance explained are shown in parentheses in the legends for corresponding antigens.

Journal: bioRxiv

Article Title: Functional Convergence of Genetically Diverse B-Cell Receptors in Simian-HIV Infected Rhesus Macaques

doi: 10.64898/2026.01.09.698730

Figure Lengend Snippet: (A) Binding activities of reference bnAbs and nnAbs (human IgG1 format) for BG505 and CH505 SOSIP and gp140 antigens were tested in standard Luminex assays using serial dilutions. Vertical dashed lines indicate the concentration point (206 ng/ml) used for calculating MFI ratios in . (B) Binding activities of rAbs (rhesus IgG1 format) derived from representative B-cell clones of different functional types were similarly tested. Results for selected rAbs from BG505-infected (top row) and CH505-infected RMs (bottom row) are shown (named as “RM_Clone-ID (Type)”). Full data for all 146 rAbs are provided in Document S3. Antigens are color-coded as in panel (A). Dashed grey curves represent MFI values detected with Luminex beads conjugated to an anti-RhIgG capture antibody. Vertical dashed lines indicate the concentration points where anti-RhIgG MFI values align closely with those detected using culture supernatants during initial screening. (C) Comparison of MFI values for autologous SOSIP and gp140 antigens detected using culture supernatants (MFI (sup)) during initial screening and rAbs (MFI (rAb)) at selected concentration points (indicated by vertical dashed lines in panel (B) and Document S3). Linear regression models were fitted, and R 2 values indicating the proportion of variance explained are shown in parentheses in the legends for corresponding antigens.

Techniques: Binding Assay, Luminex, Concentration Assay, Derivative Assay, Clone Assay, Functional Assay, Infection, Comparison

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igg1/kappa or 156 igg1/lambda isolated human myeloma plasma (Millipore)

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